Mass Spectrometry
The Mass Spectrometry Center
位于Merkert化学中心的质谱中心(CMS)设施维护着一系列最先进的质谱仪和色谱系统, 提供全套的电离和分离选项，能够处理所有样品类型和广泛的客户需求. Ionization methods available include ESI, 飞镖, 和谱技术, 所有这些都能够在正离子和负离子模式下工作.
Samples are generally run by the facility staff, and the results are provided as electronic data files. In special circumstances, 经常使用特定光谱仪的个别电子游戏正规平台人员可以接受培训并获得亲自运行样品的授权. 然后，这些获得授权的用户经常为自己电子游戏正规平台小组的其他成员运行样本，以减轻设施工作人员的压力.
如果您是外部用户，希望使用Merkert CMS设施，请与. Marek多明 to discuss your project.
请注意，在BC核心设施和充电中心进行的所有工作都应该得到适当的认可. 如果您正在发布或展示在BC核心设施和充电中心获得的数据, 请在您的手稿/海报/报告的致谢部分包括以下声明, "The authors would like to thank the Boston College
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Complete Facility Description
1. Advion Expression CMS
TThe expression CMS is a single 四极 mass spectrometer, equipped with ESI and APCI probes, switchable with minimal effort.
该仪器的质量范围为m/ z10至m/ z1200，分辨率为0.5-0.7 m/z, units at 1000 m/z, over the entire acquisition range.
在Full扫描模式下CMS对利血平的灵敏度为100pg，在SIM模式下为10pg, with a 5μl sample injection volume of 100μl/min.
2. JEOL AccuTOF 飞镖
The JEOL AccuTOF, 配置了一个新的直接分析实时(飞镖)离子源, represents a significant breakthrough in mass spectrometry. It is the first mass spectrometer to allow the analysis of solids, 只需将样品置于飞镖和质谱仪之间，即可在大气压和地电位下检测液体和气体样品. No solvents or sample preparation are required. The system design results in high throughput with no carry over, even with neat polyethylene glycol (PEG). 飞镖甚至可以直接分析各种表面(如混凝土)的样品, 货币, 食物, 药片, 服装, and TLC plates. AccuTOF 飞镖组合通过高分辨率实现了高特异性. 精确的质量测量加上精确的同位素丰度可以根据元素组成迅速识别未知化合物.
飞镖离子源是基于激发态物质与试剂分子和极性或非极性分析物的反应. 它不受其他大气压力离子源的限制, 需要将气态或汽化液体样品直接暴露在高温和电势下, ultraviolet irradiation, laser radiation, or a high-velocity gas stream. 飞镖 does not alter the sample in any way, 它也不会让使用者接触到潜在的有害物质或危险情况.
3. Agilent 6220 Time-of-Flight LC/MS
Agilent 6220 TOF LC/MS具有卓越的分析性能, including 2-ppm mass accuracy, and is ideal for protein and metabolite profiling. 与其他方法相比，强大的分辨率和高质量精度的结合可以更快，更有信心地识别复杂矩阵中的杂质.
Coupled to the mass spectrometer, 1200系列快速分辨率LC系统提供了独特的灵活性组合, resolution and speed. 优点包括能够在同一色谱系统上运行快速分辨率和常规HPLC方法, which supports both standard and narrow bore columns. Rapid resolution, 反过来, offers up to 60% higher resolution than conventional HPLC, with approximately 20 times faster analysis time, compared to standard HPLC.
4. Agilent 6460 Triple Quad
6460三重四极质谱仪是一种液相色谱三重四极质谱仪，使用三组平行棒(在这种情况下)进行质谱/质谱分析, 四极, 六极, 四极). 第一个四极将离子分离成前体离子，前体离子在六极中分裂成产物离子, which are separated by the second 四极. 经常, 采用多反应监测(MRM)模式，对两个或多个前体离子及其产物离子进行顺序监测. You can monitor up to 4000 MRM transitions by using Dynamic MRM.
The Bruker Autoflex Max
布鲁克Autoflex Max MALDI-TOF质谱仪可以分析宽m/z范围内的离子, enabling analysis of a broad spectrum of molecules, from smaller molecules, for example lipids and peptides, up to polymers and intact proteins larger than 100 kDa. With their PAN panoramic focusing and new 10 bit digitizer, peak resolution is maintained, 并增强动态范围，以更完整地观察低丰度样品成分. In MALDI MS imaging applications, 这种动态范围的扩展对于复杂的样品(如组织)是非常宝贵的, but also beneficial for all applications, including direct TLC-MALDI, and polymer characterization.
Mass spectrometry instrument training request procedure:
目前, 在完成所需的培训后，用户将可以访问JEOL AccuTOF 飞镖和Bruker MALDI系统. 培训 requests can and should be initiated via iLabs, 以便安排合适的日期和时间进行培训. 在允许进行非工作时间的工作之前，还要经过一段时间的监督.
Marek多明, MSc FRSC CChem CSci
导演, Mass Spectrometry Center
Mr. Marek多明于2005年8月加入电子游戏软件化学系. A native of England, Mr. 多明在伦敦药学院工作了十年，是一个大型质谱小组的成员. He obtained his M.Sc. degree in chemical research from Birkbeck College, where he used capillary electrophoresis, 作为气相色谱-质谱法的替代方法，用于检测羟基自由基修饰的DNA碱基. During his graduate studies, 他利用MALDI-TOF对微生物进行了表征电子游戏正规平台. Mr. Domin has been elected to the Royal Society of Chemistry, Editorial Board, for their "New Developments in Mass Spectrometry" book series, along with Professors Juan Garcia-Reyes and Frank Sobott. His research interests include the application of mass spectrometry as a routine tool for chemistry/chemical biology; exploring new research applications for mass spectrometry; reaction monitoring by mass spectrometry; and the use of open air ionization methods for the analysis of pharmaceutical, 生物, and chemical analysis.
“Ambient Ionization Mass Spectrometry” edited by Marek多明
Link to Publications
Mass Spectrometry Center
Marek多明, M.Sc.
导演
245 Beacon Street
Chestnut Hill MA 02467-3808
工作:617.552.3077
Mobile: 617-895-7739
电子邮件: domin@hjexports.net